What should be done first when creating any new smear slide?

What should be done first when creating any new smear slide? Heat the slide slightly to help the bacteria adhere. Add water to the surface of the slide. Sterilize the slide by dipping into ethanol.

What should be done first when creating any new smear slide quizlet?

What should be done first when creating any new smear slide? Label the slide with the name of the organism.

What is the correct order of steps for a smear prep?

What is the correct order for making a bacterial smear on the slide?

In order to stain the bacterial specimen for microscopy one must first prepare the smear on the slide. This basically involves three steps----transferring a liquid suspension of the bacterium on the slide, drying the smear, and then heating slightly to firmly attach the smear to the slide.

How do you prepare a smear slide on a microscope?

Culture from solid media: Using a sterile pipette, add one drop of sterile saline or sterile water to the center of the microscope slide. Aseptically pick a small amount of an isolated colony with a loop and gently mix into the drop of sterile saline or water using circular motions. Mix evenly to make a thin smear.

Hematology- Making a Peripheral Blood Smear

How do you prepare a slide?

What aseptic techniques are used when making a smear prep?

What is the correct sequence for making a specimen smear on a slide quizlet?

Place the following steps in the correct sequence:1-Staining; 2-Making a smear; 3-Fixing. Simple staining is often necessary to improve contrast in this microscope. Which microscope achieves the highest magnification and greatest resolution? In this microscope, the observer does NOT look at an image through a lens.

What is involved in making a smear quizlet?

Terms in this set (11)

A sample of a bacterial colony must be mixed into water or saline. Using a pipette, apply a small drop of the bacterial fluid to a clean glass slide. Smear the fluid evenly across the surface of the slide with the edge of a cover slip. Allow slide to air dry.

How are bacterial smears created?

First, bacteria are placed on a glass slide and “fixed” with heat, partly to ensure that they remain attached to the glass. Then, for simple staining, a basic stain is applied. Such a stain carries a positive electrical charge.

What is smear slide?

Smear slides samples are a rapid, simple and cheap way of examining marine sediments, and are essential for identifying sediment types and classifying sediments. As only a very small sample size is required (1-2 mm3), even archive cores can be sampled without damage.

What do you do before you put the stain under the microscope?

When making a bacterial smear on a slide you should first dilute the bacteria in water if?

When preparing a smear the bacteria on the slide should be heat fixed before the smear fully dries?

In order to heat fix a bacterial smear, it is necessary to first let the bacterial sample air dry. Then either place the slide in the slide holder of a microincinerator, or pass the dried slide through the flame of a Bunsen burner 3 or 4 times, smear side facing up. Once the slide is heat fixed, it can then be stained.

Which of the following is appropriate when creating a smear in this fashion?

Which of the following is appropriate when creating a smear in this fashion? Using a loop, you should disperse the bacterial sample material into a water drop on the slide.

How do you prepare a smear from a broth culture?

What is smear quizlet?

Terms in this set (15)

A dried preparation of bacterial cells on a glass slide is called a. smear.

Why must a heat fixed smear be made before the staining technique is performed?

Heat fixing kills cells, and adheres them to the slide. Cells will be rinsed off the slides if they are not heat fixed properly.

Why is aseptic technique important during laboratory activities like smear preparation?

Why is aseptic technique important during laboratory activities like smear preparation? Proper aseptic technique aids in keeping microbes from spreading to other surfaces, where they might be contacted by others in the lab.

What is the correct order to make a smear for a Gram stain?

The performance of the Gram Stain on any sample requires 4 basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram's Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with ...

What is the correct ordering to order to stain a microbe?

Place the steps for preparing a simple stain in the correct order: -Evenly spread bacteria across slide to make a thin smear. -Apply stain to slide for 1 to 2 minutes. -Rinse stain off slide water. -Aseptically obtain a small loop of bacteria and mix with water.

What is used first to apply bacteria to the slide in capsular staining?

The Schaeffer-Fulton method uses malachite green stain, which is applied to the bacteria fixed to a slide. The slide is then washed with water before being counterstained with Safranin. Vegetative cells will appear pinkish-red, while any endospores present will appear green.

What are the steps of aseptic technique?

What is the process of aseptic technique?

Aseptic technique means using practices and procedures to prevent contamination from pathogens. It involves applying the strictest rules to minimize the risk of infection. Healthcare workers use aseptic technique in surgery rooms, clinics, outpatient care centers, and other health care settings.

What are the precautionary measures to be observed before during and after applying the aseptic culture technique?