What is the purpose of a smear?

The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium. Below are some guidelines for preparing a smear for a Gram-stain

Gram-stain

Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884.

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Gram stain - Wikipedia

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What is the purpose of the smear quizlet?

What is the purpose of a bacterial smear? Prepare a sample of bacteria that adheres to a slide. The sample can then be stained so as to better view the bacteria.

What are 2 purposes served by preparing a smear?

The purpose of making a smear is to fix the bacteria onto the slide. Fixing the bacteria will preserve the morphology of the cells long-term. Also, fixation assists the cells in adhering to the slide, so that the cells do not fall off the slide during the staining procedure.

What is a smear in microbiology?

A bacterial smear is a thin layer of bacteria placed on a glass blade and «fixed» by heat or other, partly to ensure that they remain attached to the glass, for coloring. It can be prepared from a solid medium or broth. Then a coloring is applied.

What is a smear preparation?

In a smear preparation, cells from a culture are spread in a thin film over a small area of a microscope slide, dried, and then fixed to the slide by heating or other chemical fixatives. A good smear preparation is the key to a good stain.

What is the purpose of a smear campaign when divorcing or breaking up???

How many types of smears are there?

Four different types of smear preparation methods (conventional method, blood film method, drop and rest method, and water-wash method) were carried out according to the standard reference as described below. A drop of sample was placed onto a clean slide and then spread to make a smear of 1 cm diameter.

What are bacterial smears used to examine?

The smear is a means of estimating the number of acid-fast bacteria present, reported as the Bacterial Index (BI), and is important in determining the type and severity of disease as well as assessing the response to treatment.

What is the purpose of fixing the smear that is to be stained?

This process is called heat fixing the specimen to the slide. Its purpose is to bind the specimen to the slide so that it does not wash off during staining. Killing the cells with heat fixation also increases their permeability to the dyes used in staining.

What is the significance of smear fixation?

Fixation of Cells (Smear) On the Slides:

After preparation of a uniform smear, it must be fixed or stuck over the slide. Fixation is the process by which internal and external structures of cells and micro-organisms are preserved and fixed in position.

Are bacterial smears used to observe microorganisms?

Making a bacterial smear prepares the bacteria to be stained and a simple stain is a quick and easy way to observe bacteria.

Is a smear considered a sterile procedure?

Wire loops, also known as inoculation or smear loops, are frequently used in microbiology for the purposes of transfer and culture of microorganisms. They are sterilized by flaming over a Bunsen burner, being heated to glowing red, in order to ensure that all microorganisms present on the loop are destroyed.

What is smear quizlet?

Terms in this set (15)

A dried preparation of bacterial cells on a glass slide is called a. smear.

What is the purpose of allowing the bacterial smear to dry?

What is the purpose of allowing the bacterial smear to dry once it has been placed onto the surface of the slide? Drying helps remove excess water to ensure optimal heat fixation. Air-drying adheres the bacteria to the slide.

What happens if smear is too thin?

Too thin? (can occur with anemic patients) = Increase the angle of the slide you are using to smear with; otherwise your smear may run right off of your horizontal slide and you will not have a nice feathered edge, and your smear will be so thin you will have trouble finding enough cells.

What is smear in biology?

(smîr) n. Biology A sample, as of blood or bacterial cells, spread on a slide for microscopic examination or on the surface of a culture medium.

What is tissue smear?

Definition. Small bowel tissue smear is a lab test that checks for disease in a sample of tissue from the small intestine.

What is the purpose of steaming the bacterial smear before staining?

Steam helps to loosen up the waxy layer and promotes entry of the primary stain inside the cell. The smear is then rinsed with a very strong decolorizer, which strips the stain from all non-acid-fast cells but does not permeate the cell wall of acid-fast organisms.

What is involved in making a smear quizlet?

Terms in this set (11)

A sample of a bacterial colony must be mixed into water or saline. Using a pipette, apply a small drop of the bacterial fluid to a clean glass slide. Smear the fluid evenly across the surface of the slide with the edge of a cover slip. Allow slide to air dry.

Why do you need to mark the slide before you make a smear?

Preparing a smear

It causes bacteria to adhere to a slide so that they can be stained and observed. It also kills them, rendering pathogenic bacteria safe to handle. An objective in preparing smears is to learn to recognize the correct density of bacteria to place on the slide.

What diseases require a peripheral blood smear and why?

How long does it take to get results from a blood smear test?

The blood smear is a quick test. Your doctor may draw blood from your arm or by pricking your finger. You usually get the results in one to two days.

What does a normal blood smear look like?

Due to the hemoglobin inside the RBCs, they appear pink to red in color with a pale center after staining the blood smear. When the appearance of RBCs (RBC morphology) is normal, it is often reported as normochromic (normal color) and normocytic (normal size).

How do you prepare and fix a smear?

The first step in most bacterial staining procedures is the preparation of a smear. In a smear preparation, cells from a culture are spread in a thin film over a small area of a microscope slide, dried, and then fixed to the slide by heating or other chemical fixatives.