What is pathology decalcification?

Decalcification describes the technique for removing minerals from bone or other calcified tissue so that good-quality paraffin sections can be prepared that will preserve all the essential microscopic elements.

What is the purpose of decalcification?

Decalcification is routinely used technique in most histopathological laboratories for the microscopic examination of calcified tissues [4]. The purpose of decalcification is to remove calcium salts from mineralized tissue, resulting in preservation of organic components [5].

What is the meaning of decalcification?

Definition of decalcification

: the removal or loss of calcium or calcium compounds (as from bones or soil)

What are the types of decalcification?

Six decalcifying agents namely, neutral ethylene diamine tetra acetic acid (EDTA) decalcifying solution, 5% nitric acid, Perenyi's fluid, formalin–nitric acid, 5% trichloracetic acid, and 10% formic acid were used to decalcify 24 natural teeth (four in each solution).

What are the method of decalcification?

The most common acids used for decalcification are 5-10% solutions of hydrochloric acid (HCl), nitric acid, and formic acid. These acids can be used alone or in combinations. The following should be considered before implementing an acid decalcifying protocol in the laboratory.

Decalcification

What is the principle of decalcification?

The principle of this decalcification method is to obtain complete decalcification by a mixture of as high pH as possible without diminishing the stainability of the Nissl-granules (with Einarson's progressive staining method by means of gallocyanin).

What are decalcifying agents?

Decalcifying agents are acidic substances that combine with lime in bone salts, teeth, chitin, etc., forming water-soluble compounds that easily can be removed.

What causes decalcification of bones?

The Loss of calcium from the bones is the major cause of decalcification. With the loss of density, bones become brittle and fragile, increasing the risk of bone fracture.

How can I make my pineal gland healthy?

Create an environment that promotes the proper functioning of the pineal gland: Getting high-quality sleep every night is crucial for optimal functioning. One factor that affects your sleep quality is blue light. It suppresses the production of melatonin for up to four hours.

How is decalcification of tissue done?

Place the tissue in a 10% formic acid solution until tissue is determined to be decalcified. This could take anywhere from a few hours to several days. Use a large volume of solution to tissue. You should change the solution daily if the decalcification process takes more than one day.

What vitamins are good for the pineal gland?

These top seven supplements: apple cider vinegar, boron/ borax, iodine, turmeric, raw cacao, chaga mushroom and oregano oil have the potential to detoxify or decalcify, strengthen and stimulate the pineal gland to work better.

How do you reverse pineal gland calcification?

Because most doctors don't recognize a calcified pineal gland as a medical issue, there aren't medical treatments to reduce calcium deposits on the pineal gland. Most changes are related to a person's food intake and chemical or sun exposure.

What stimulates the pineal gland?

Light exposure is the most important factor related to pineal gland function and melatonin secretion.

What is the best and safest treatment for osteoporosis?

Bisphosphonates are usually the first choice for osteoporosis treatment. These include: Alendronate (Fosamax), a weekly pill. Risedronate (Actonel), a weekly or monthly pill.

What are 3 common causes of osteoporosis?

What are the early warning signs of osteoporosis?

However, some signs and symptoms, such as receding gums, weaker grip strength, and more brittle fingernails may be early warning signs. A loss of height, a stooped posture, back or neck pain, and bone fractures are often the most common symptoms of later-stage osteoporosis.

What is the best decalcifying agent?

Formic acid with gentle agitation produced the best decalcification overall based on time required for decalcification, ease of sectioning, hard and soft tissue staining and tissue preservation.

How do you test for decalcification?

Chemical method:

Calcium oxalate forms creating a cloudy precipitate if it has calcium to bind with. If solution is cloudy, tissue is still releasing calcium into decal solution. Decal solution should be changed and tissue should continue to decalcify. If solution is clear, decalcification is complete.

What frequency opens the pineal gland?

The track is tuned to 963 Hz, which has the potential to enhance your meditation to activate your pineal gland. Activating your pineal gland can be an immerse experience and it will take patience and practice.

Why pineal gland is known as third eye?

The name “third eye” comes from the pineal gland's primary function of 'letting in light and darkness', just as our two eyes do. This gland is the melatonin-secreting neuroendocrine organ containing light-sensitive cells that control the circadian rhythm (1).

What two hormones does the pineal gland produce?

Pineal hormones

Both melatonin and its precursor, serotonin, which are derived chemically from the alkaloid substance tryptamine, are synthesized in the pineal gland. Along with other brain sites, the pineal gland may also produce neurosteroids.

What happens if your pineal gland is calcified?

Morphological changes associated with pineal calcification include changes in the production of melatonin, due to the decreased function in the pineal gland parenchyma, and results in decreased pineal volume, reduced melatonin production in humans [14], and altered sleep patterns [59].

What happens when the pineal gland is activated?

Stimulation of the pineal gland via its sympathetic innervation pathway results in the production of N-acetylserotonin and melatonin. Melatonin has many therapeutic roles and is heavily implicated in the regulation of the sleep-wake cycle.

What are the disorders of the pineal gland?

What foods heal the pituitary gland?

Diet for healthy Pituitary gland

Important nutrients for good pituitary function are a variety of minerals- especially manganese, magnesium, and vitamin E. Foods rich in minerals are wheat, leafy greens, nuts and some legumes. Other important nutrients are iron and iodine.