What feature of protein structure is most directly responsible for enzyme substrate specificity?

What feature of protein structure is most directly responsible for this specificity? B-Most protein enzymes have rigid, static structures essential to their specificity. The active site is the location in an enzyme where substrates (reactants) bind and where catalysis occurs.

Which level of protein structure is most directly responsible for enzyme specificity?

Tertiary structure is the most important of the structural levels in determining, for example, the enzymatic activity of a protein.

How protein structure is involved in enzyme specificity?

Explain how protein structure is involved in enzyme specificity. Enzymes are proteins, and proteins are macromolecules with unique three-dimensioal configuration. The specificity of an enzyme results from its shape, which is a consequence of its amino acid sequence.

What determines the specificity of a protein enzyme for a particular substrate?

The most important factor governing the fit of a substrate for an enzyme is the amino acid sequence around the bond to be cleaved.

What part of an enzyme determines its specificity?

The apoenzyme is responsible for the enzyme's substrate specificity.

Enzymes (Updated)

What is the structural basis for enzyme specificity?

What is the structural basis for enzyme specificity? The intricate three-dimensional structure of proteins allows the construction of active sites that will recognize only specific substrates.

What is protein specificity?

Specificity describes the strength of binding between a given protein and ligand. This relationship can be described by a dissociation constant, which characterizes the balance between bound and unbound states for the protein-ligand system.

What is substrate specificity?

Definition. A feature of enzyme activity with regard to the kind of substrate reacting with an enzyme to yield a product. Supplement. In an enzyme activity, the substrate must bind with the enzyme to become a catalyst of a chemical reaction.

How do you find the substrate specificity?

Traditionally, the general method of evaluating the specificity of a given enzyme is to measure the specificity constant of a group of substrates individually followed by comparison of measured values, regardless of the assay used for measurement 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15.

Which of the following is a substrate specific enzyme?

1 Answer. Lactase is a substratespecific enzyme.

What determines the specificity of an enzyme Mcq?

Explanation: Specificity refers to discrimination of an enzyme between two competing substrates. They are three types of specificities as given below: Substrate specificity, reaction specificity and stereo specificity. There nothing exists as Koshland specificity.

Which of the following statements best helps explain the reaction specificity of an enzyme?

Which of the following statements best helps explain the reaction specificity of an enzyme? The shape and charge of the substrates are compatible with the active site of the enzyme.

Why is the tertiary structure of a protein important?

The Significance of Protein Structure

Their tertiary structure gives proteins a very specific shape and is an important feature in the 'lock and key' function of enzymes, or receptor sites on cell membranes. Specificity can vary, in some cases sites can allow some variation in structure in other cases not.

What is responsible for each enzyme having the specificity for interacting with only a single substrate?

That's why a particular enzyme by virtue of its specificity recognizes and reacts with its particular substrate. Binding and catalytic sites are collectively called active site . Binding site binds the specific substrate. Catalytic site transforms the specific substrate into products.

What is the tertiary structure of protein dependent on?

The overall three-dimensional structure of a polypeptide is called its tertiary structure. The tertiary structure is primarily due to interactions between the R groups of the amino acids that make up the protein.

What are enzymes specificity?

Specificity is defined as the ability of an enzyme to choose an exact substrate from a group of the same chemical molecules. Actually, specificity is a molecular recognition mechanism that works through complementarity in conformation and structure between the enzyme and the substrate.

How do you find the substrate of an enzyme?

One way to identify potential protease substrates is to determine the peptide sequences they cleave in vitro, in other words, which amino acids span the cleavage site and are recognized by the enzyme's active site. These sequences are then used, like partial license plate numbers, to search the proteome for substrates.

What determines an enzyme's function?

Enzyme activity can be affected by a variety of factors, such as temperature, pH, and concentration. Enzymes work best within specific temperature and pH ranges, and sub-optimal conditions can cause an enzyme to lose its ability to bind to a substrate.

Why is an enzyme-substrate specific?

Enzymes are specific to substrates as they have an active site which only allow certain substrates to bind to the active site. This is due to the shape of the active site and any other substrates cannot bind to the active site.

How does the active site promotes enzyme-substrate specificity?

The active site of an enzyme is very specific to its substrates as it has a very precise shape. This results in enzymes being able to catalyze only certain reactions as only a small number of substrates fit in the active site. This is called enzyme-substrate specificity.

What best explains the observation of substrate specificity?

What best explains the observation of substrate specificity? There is a precise compatibility between an enzyme's active site and the substrate molecule.

Which enzyme has absolute specificity for its substrate?

The enzyme glutamate dehydrogase (1BGV. pdb) will catalyze only the removal of the nitrogen group from glutamate- NOT any other amino acid. It has absolute specificity.

Why does the activation energy of a reaction not appear in the final DG of the reaction?

Give with one hand, take with the other. Why does the activation energy of a reaction not appear in the final Delta G of the reaction? 7. The energy required to reach the transition state (the activation energy) is returned when the transition state proceeds to product.

How does tertiary structure affect enzyme?

If the enzyme accepts the substrate, the reaction takes place and the active site changes to fit the substrate. If its tertiary structure is changed too much, however, the active site of an enzyme will not accept the substrate, and the chemical reaction will not take place.

Which structure of proteins enables more of enzymatic nature?

The activity of enzymes is dependent upon the maintenance of tertiary structure of the protein component.