Is Mycobacterium Phlei acid-fast?

Mycobacterium phlei is a species of acid-fast bacteria in the genus Mycobacterium. It is characterized as one of the fast-growing mycobacteria.

Is Mycobacterium Phlei gram negative?

Bacterial strains tested included the gram positive strains: Staphylococcus aureus methicillin-sensitive, Enterococcus faecalis, Mycobacterium phlei, Bacillus subtilus, and the gram negative strains: Eschericia coli wild strain, Pseudomonas aeruginosa 187 (wild).

Is M Phlei Bacillus?

The grass bacillus, Mycobacterium phlei, was first described in 1898–1899 as a member of the order Actinomycetales and it is found in the environment (Gordon and Smith 1953; Wayne et al. 1969; Stackebrandt et al. 1981).

Where is Mycobacterium Phlei found?

Mycobacterium phlei is a fast-growing, saprophytic bacterium that is widely distributed in soil and dust and on plants. It has only occasionally been associated with disease in humans with a suppressed immune system (5–7, 9) or in immunocompetent individuals (4, 8, 10).

Is smegmatis acid-fast?

The small pink bacilli above are Mycobacterium smegmatis, an acid fast bacteria because they retain the primary dye.

ACID FAST STAINING | Acid fast organisms | Bacterial Staining Technique | Microbiology

Is Mycobacterium acid-fast positive or negative?

Acid-fast bacteria are gram-positive, but in addition to peptidoglycan, the outer membrane or envelope of the acid-fast cell wall of contains large amounts of glycolipids, especially mycolic acids that in the genus Mycobacterium, make up approximately 60% of the acid-fast cell wall (Figure 2.3C. 2).

Is Mycobacterium tuberculosis acid-fast?

Sputum, or phlegm, is often used to test for Mycobacterium tuberculosis, to find out if a patient has TB. This bacterium is completely acid-fast, which means the entire cell holds onto the dye.

Is Mycobacterium Phlei motile?

Size: 0.2-0.8 micrometers by 1.0-10.0 micrometers. Motility: Non-motile.

Is Staphylococcus epidermidis acid fast stain?

The darker staining cocci are Staphylococcus epidermidis , a non-acid fast bacterium.

What is the arrangement of Mycobacterium Phlei?

These ridges consists of rounded bodies which are made up of clustered cells. The top of the cone consists of small, rugged, irregular structures. Thin sections show that the cells are arranged close together, without interconnections but with some electron-dense material between them.

What does Mycobacterium Phlei look like?

phlei is a rod-shaped bacterium 1.0 to 2.0 micrometers in length. If grown on an agar plate, M. phlei colonies appear orange to yellow in color, and predominantly dense with smooth edges, although some smaller filamentous colonies have also been described. Like other mycobacteria, M.

Is Staphylococcus aureus acid-fast positive?

Mycobacterium sp. (acid-fast rods) and Staphylococcus aureus (non-acid-fast cocci).

Are acid-fast negative cells stained by Carbolfuchsin?

Are acid-fast negative cells stained by carbolfuchsin? If so, how can this be a differential stain? Yes they are initially stained by carbolfuchsin but the decolorizing step, acid alcohol, removes stain from acid fast negative cells while acid fast positive cells retain the stain.

Is Bacillus subtilis acid-fast?

Therefore, it was noted that B. subtilis was acid-fast negative, whereas M. smegmatis was acid-fast positive. The acid-fast stain is classified under differential staining procedures that are used to pick out acid-fast organisms, including bacteria of the genus Mycobacterium.

Does Mycobacterium Phlei produce endospores?

We conclude that it is unlikely that Mycobacterium is capable of endospore formation.

Is Micrococcus luteus acid-fast?

A photomicrograph of Mycobacterium smegmatis (pink) and Micrococcus luteus (blue) at 1000x magnification. M. smegmatis is acid-fast, retaining the carbol fuchsin dye, thus appearing pink.

What makes Mycobacterium resistant to staining?

What makes Mycobacterium resistant to staining? Mycobacterium has a waxy cell wall that contains mycolic acid, a complex lipid that prevents stains from penetrating the cell.

What is the Gram stain reaction for Staphylococcus epidermidis?

Staphylococcus epidermidis is a gram-positive and coagulase-negative staphylococci, and one of over 40 species belonging to the genus Staphylococcus. It is part of the normal human flora, typically the skin flora, and less commonly the mucosal flora.

What Gram stain is Staphylococcus epidermidis?

Staphylococcus epidermidis is a coagulase-negative, gram-positive cocci bacteria that form clusters.

What are acid-fast bacteria?

Introduction. Acid-fast bacteria, also known as acid-fast bacilli or simply AFB, are a group of bacteria sharing the characteristic of acid fastness. Acid fastness is a physical property that gives a bacterium the ability to resist decolorization by acids during staining procedures.

Is Mycobacterium non-motile?

Mycobacteria are Gram-positive, catalase positive, non-motile, non-spore forming rod-shaped bacteria (0.2–0.6 μm wide and 1.0–10 μm long).

Why is Mycobacterium tuberculosis non-motile?

Mycobacterium tuberculosis is a nonmotile, non-spore-forming, obligate aerobe, acid-fast bacillus that often appears beaded or unstained using Gram stain. Like all mycobacteria, it is distinguished by its ability to form stable mycolate complexes with arylmethane dyes (carbolfuchsin, auramine, and rhodamine).

What are examples of non acid-fast bacteria?

Why is Mycobacterium called acid-fast bacteria?

Mycobacterium and many Nocardia species are called acid-fast because during an acid-fast staining procedure they retain the primary dye carbol fuchsin despite decolorization with the powerful solvent acid-alcohol. Nearly all other genera of bacteria are nonacid-fast.

Is Mycoplasma acid-fast?

This organism is however, the most common cause of atypical pneumonia. Symptoms include a persistant cough, little to no sputum production, infiltrates on chest radiographs, and little toxicity (walking pneumonia). The organism does not produce a cell wall and is not stainable by gram stain or by acid-fast stain.