Is Corynebacterium acid-fast?

Etiology. Corynebacterium spp., members of the family Corynebacteriaceae, are Gram-positive, non-spore- forming, non-motile, aerobic, pleomorphic rods with coccoid or club-shaped appearance that are catalase-positive and non-acid-fast (Boone et al., 2001; Greenwood, 2007).

What are the characteristics of Corynebacterium?

The principal features of the genus Corynebacterium were described by Collins and Cummins in 1986. They are gram-positive, catalase-positive, non-spore-forming, non-motile, rod-shaped bacteria that are straight or slightly curved. Metachromatic granules are usually present representing stored phosphate regions.

Is Corynebacterium diphtheriae acid-fast positive or negative?

C. diphtheriae is a pleomorphic, club-shaped, Gram positive bacillus that is catalase positive, non-motile, non-spore forming, and non-acid fast.

Does Corynebacterium have Mycolic acid?

Most Corynebacterium species contain mycolic acids with 22 - 36 carbons, although ranges of 20 − 38 carbons have also been detected.

What are examples of acid-fast bacteria?

Acid-Fast Stain

What are examples of non acid-fast bacteria?

Which genus contains acid-fast bacteria?

The acid-fast stain is a differential stain used to identify acid-fast organisms such as members of the genus Mycobacterium . Acid-fast organisms are characterized by wax-like, nearly impermeable cell walls; they contain mycolic acid and large amounts of fatty acids, waxes, and complex lipids.

Does Corynebacterium Diphtheriae have Mycolic acid?

As a member of the CMNR group, C. diphtheriae is closely related to members of the genera Mycobacterium, Nocardia and Rhodococcus. Almost all members of these genera comprise an outer membrane layer of mycolic acids, which is assumed to influence host-pathogen interactions.

What is structure of Corynebacterium?

Corynebacterium species are characterized by a complex cell wall architecture: the plasma membrane of these bacteria is followed by a peptidoglycan layer, which itself is covalently linked to a polymer of arabinogalactan.

Is Escherichia acid fast?

Escherichia coli is a NON ACID-FAST bacterium. (1) Bacteria are DECOLORIZES by ACID ALCOHOL and DO NOT retain the initial stain, carbolfuchsin, (2) so it can pick up the counterstain, METHYLENE BLUE.

Is Corynebacterium Xerosis acid-fast?

Abstract. The genus Corynebacterium is composed of a wide variety of Gram-positive, non-acid-fast, nonmotile, rod-shaped, catalase-positive bacteria. The bulk of the species is considered to be normal cutaneous, pharyngeal and gastrointestinal flora of humans.

Is Corynebacterium beta hemolytic?

Arcanobacterium haemolyticum, formerly known as Corynebacterium hæmolyticum, is a gram-positive, catalase-negative, aerobic, beta-hemolytic and non-mobile bacillus.

Is Corynebacterium Gram-positive or negative?

Corynebacterium diphtheriae is a Gram-positive nonmotile, club-shaped bacillus. Strains growing in tissue, or older cultures in vitro, contain thin spots in their cell walls that allow decolorization during the Gram stain and result in a Gram-variable reaction.

Is Corynebacterium a lactic acid bacteria?

Corynebacterium crenatum cells with sustained acid production ability and high acid volumetric productivity harvested from the glutamic acid fermentation broth were used to produce succinic acid and lactic acid.

Is Corynebacterium aerobic or anaerobic?

Corynebacteria (from the Greek words koryne, meaning club, and bacterion, meaning little rod) are gram-positive, catalase-positive, aerobic or facultatively anaerobic, generally nonmotile rods.

Is Corynebacterium is facultative anaerobic?

Members of the genus Corynebacterium are gram-positive, small, pleomorphic, and nonsporing rods. They are nonmotile, usually facultatively anaerobic, and fermentative.

What is composed of Mycolic acids?

Mycolic acids are major and specific lipid components of the mycobacterial cell envelope and are essential for the survival of members of the genus Mycobacterium that contains the causative agents of both tuberculosis and leprosy.

Is diphtheria aerobic or anaerobic?

C. diphtheriae is an aerobic, gram-positive bacillus. Toxin production (toxigenicity) occurs only when the bacillus is itself infected (lysogenized) by specific viruses (corynebacteriophages) carrying the genetic information for the toxin (tox gene).

How do you identify Corynebacterium diphtheriae?

Corynebacterium Infections☆

diphtheriae, each differentiated by hemolysis, colonial morphology, and fermentative reactions. Diagnosis is made by identifying the microorganism in cultures from swabs of the nasopharynx or skin lesions. The specimens can be plated on Löeffler's or cysteine tellurite agar media.

What is no acid-fast bacilli seen?

A normal result for an acid-fast bacteria smear is negative, meaning no bacteria were found in the sputum sample. A positive result means that bacteria were found and that you may have an infection. The smear is treated with a special acid-fast stain that can provide a preliminary test result in 24 hours.

Is Staphylococcus aureus acid-fast?

Acid-fast rods and non-acid-fast cocci

(acid-fast rods) and Staphylococcus aureus (non-acid-fast cocci).

What makes a microorganism non acid-fast?

What makes a microorganism non-acid-fast? Microbes that lack myolic acid (which contains 60% lipids) are non-acid-fast.

Is Klebsiella pneumoniae acid-fast?

acid fast stain. The small pink bacilli above are Mycobacterium smegmatis, an acid fast bacteria because they retain the primary dye. The darker staining cocci are Staphylococcus epidermidis , a non-acid fast bacterium. The capsule of Klebsiella pneumoniae is demonstrated here.

Why acid-fast bacilli are called acid-fast?

Mycobacteria are called acid-fast bacilli because they are rod-shaped bacteria (bacilli) that can be seen under the microscope following a staining procedure in which the bacteria retain the color of the stain after an acid wash (acid-fast).

Is Bacillus subtilis acid-fast?

Therefore, it was noted that B. subtilis was acid-fast negative, whereas M. smegmatis was acid-fast positive. The acid-fast stain is classified under differential staining procedures that are used to pick out acid-fast organisms, including bacteria of the genus Mycobacterium.